DNA ligases seal five′-PO4 and 3′-OH polynucleotide finishes via three nucleotidyl transfer methods involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic condition syndromes. Crystal constructions of DNA ligases certain to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases identify harmed DNA finishes, and how protein domain movements and active-site remodeling are utilized to choreograph the tip-joining pathway.
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